State of the art lentiviral vector manufacturing

Lentiviral Vector

A retroviral vector system based on the human immunodeficiency virus (HIV) was developed that, in contrast to a murine leukemia virus-based counterpart, transduced heterologous sequences into HeLa cells and rat fibroblasts blocked in the cell cycle, as well as into human primary macrophages. Additionally, the HIV vector could mediate stable in vivo gene transfer into terminally differentiated n...

Special Issue: AI in Manufacturing: State of the Art

The AAAI Special Interest Group on AI in Manufacturing ~SIGMAN! organization represents a wide array of researchers addressing fundamental scientific problems that arise in engineering design and manufacturing. The human endeavor that is design and manufacturing is rich in basic problems for researchers in Artificial Intelligence. From process planning researchers who aim to augment the abiliti...

Enzyme Immobilization: The State of Art in Biotechnology

The advantages of immobilized enzyme over its soluble counterpart arise from their improved stability andeasy separation from the reaction media, leading to decrease in production cost. Immobilization methodsrange from adsorption onto matrices, entrapment, cross-linking and covalent bonding to prefabricatedcarriers or activated supports. Changes in kinetic properties of immobi...

State-of-the-art lentiviral vectors for research use: risk assessment and biosafety recommendations.

Lentiviral vectors (LV) are competent gene transfer vehicles, as used for both research and gene therapy applications, because of their stable integration in non-dividing and dividing cells and long-term transgene expression. Along with our understanding that LV offer solutions for gene therapy, biosafety concerns have uncovered risks due to insertional mutagenesis, the generation of replicatio...

Gene Regulatable Lentiviral Vector System